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Magnetic targeting and ultrasound mediated drug delivery: Benefits, limitations and combination. The development of non-viral gene-activated matrices for bone regeneration using polyethyleneimine PEI and collagen-based scaffolds. Animal Models for Vaccine Therapy. The association of autophagy with polyethylenimine-induced cytotoxity in nephritic and hepatic cell lines. Gellan gum blended PEI nanocomposites as gene delivery agents: Evidences from in vitro and in vivo studies.

Cyclic RGD-poly ethylene glycol -polyethyleneimine is more suitable for glioblastoma targeting gene transfer in vivo. Co-delivery of anti-vascular endothelial growth factor siRNA and doxorubicin by multifunctional polymeric micelle for tumor growth suppression. Application of Magnetic Nanoparticles to Gene Delivery. Biocompatible Polymers for Nucleic Acid Delivery.

Use of polyethylenimine-modified magnetic nanoparticles for highly specific enrichment of phosphopeptides for mass spectrometric analysis. Lifelong reporter gene imaging in the lungs of mice following polyethyleneimine-mediated sleeping-beauty transposon delivery. Novel role and mechanism of protein inhibitor of activated STAT1 in spatial learning.

Modified polyethyleneimine with histidine—lysine short peptides as gene carrier. Metabolically stabilized long-circulating PEGylated polyacridine peptide polyplexes mediate hydrodynamically stimulated gene expression in liver. Two and three-dimensional gene transfer from enzymatically degradable hydrogel scaffolds. Poly ethylene glycol analogs grafted with low molecular weight poly ethylene imine as non-viral gene vectors. Synthesis and characterization of dexamethasone-conjugated linear polyethylenimine as a gene carrier.

Dosage Formulation. Investigation of gene transferring efficacy through nano-polyplex consisting of methylated N- 4-pyridinylmethyl chitosan chloride and poly ethylenimine in human cell lines. Endosomal disruptors in non-viral gene delivery. Preparation and characterization of polyethylenimine-coated Fe 3 O 4 -MCM nanocomposite particles as a novel agent for magnet-assisted transfection. Cost-effective gene transfection by DNA compaction at pH 4. Polyethylenimine nanoparticles as an efficient in vitro siRNA delivery system.

Women and heart disease — Physiologic regulation of gene delivery and expression: Bioreducible polymers and ischemia-inducible gene therapies for the treatment of ischemic heart disease. DNA delivery from matrix metalloproteinase degradable poly ethylene glycol hydrogels to mouse cloned mesenchymal stem cells. Pathological and protective roles of glia in chronic pain.

Gene delivery systems for gene therapy in tissue engineering and central nervous system applications. Galactose-poly ethylene glycol -polyethylenimine for improved lung gene transfer. Controlled complexation of plasmid DNA with cationic polymers: Effect of surfactant on the complexation and stability of the complexes. Polyhydroxyethylaspartamide-spermine copolymers: Efficient vectors for gene delivery.

Characterization of the transgene expression generated by branched and linear polyethylenimine-plasmid DNA nanoparticles in vitro and after intraperitoneal injection in vivo. Poly lactic-co-glycolic acid nanosphere as a vehicle for gene delivery to human cord blood-derived mesenchymal stem cells: comparison with polyethylenimine. Nonviral gene transfection nanoparticles: function and applications in the brain. Serum- and glucocorticoid-inducible kinase 1 enhances zif expression through the mediation of SRF and CREB1 associated with spatial memory formation.

Poly ethylene glycol -block-polyethylenimine copolymers as carriers for gene delivery: Effects of PEG molecular weight and PEGylation degree. Nanosphere-mediated delivery of vascular endothelial growth factor gene for therapeutic angiogenesis in mouse ischemic limbs. Analysis of the intracellular barriers encountered by nonviral gene carriers in a model of spatially controlled delivery to neurons. Determination of nanoparticle vehicle unpackaging by MR imaging of a T2 magnetic relaxation switch.

SATB2 interacts with chromatin-remodeling molecules in differentiating cortical neurons. Macromolecular Drug Delivery. Progress and Prospects in Ocular Gene Therapy. Antifreeze Properties of Polyglycidol Block Copolymers. Poly 2-oxazolines in biological and biomedical application contexts.

Molecular design of functional polymers for gene therapy. Derivatizing weak polyelectrolytes—Solution properties, self-aggregation, and association with anionic surfaces of hydrophobically modified poly ethylene imine. Self-complementary sequences induce the formation of double-stranded filamentous phages. Efficacy of nonviral gene transfer in the canine brain. Influence of acyl chain length on transfection mediated by acylated PEI nanoparticles. Non-viral gene delivery: from the needle to the nucleus.

Polymer based cardiovascular gene therapy. Serum- and glucocorticoid-inducible kinase1 enhances contextual fear memory formation through down-regulation of the expression of Hes5. Targeted polymeric gene delivery for anti-angiogenic tumor therapy. Gene Therapy. Polyethyleneimine functionalized iron oxide nanoparticles as agents for DNA delivery and transfection.

DNA delivery to the mitochondria sites using mitochondrial leader peptide conjugated polyethylenimine. Polyethylenimine—DNA solid particles for gene delivery. A multifunctional PEI-based cationic polyplex for enhanced systemic pmediated gene therapy. PEGylated gene nanocarriers based on block catiomers bearing ethylenediamine repeating units directed to remarkable enhancement of photochemical transfection.

An efficient GLP-1 expression system using two-step transcription amplification. Optimising non-viral gene delivery in a tumour spheroid model. Emerging vectors and targeting methods for nonviral gene therapy. Intraperitoneal linear polyethylenimine L-PEI -mediated gene delivery to ovarian carcinoma nodes in mice. Barriers to carrier mediated drug and gene delivery to brain tumors. A versatile family of degradable non-viral gene carriers based on hyperbranched poly ester amine s.

Gene delivery with low molecular weight linear polyethylenimines. Recent advances in rational gene transfer vector design based on poly ethylene imine and its derivatives. Polyethylenimine-based non-viral gene delivery systems.

Cancer gene discovery in solid tumours using transposon-based somatic mutagenesis in the mouse. Design and development of polymers for gene delivery. Quantitative comparison of polyethylenimine formulations and adenoviral vectors in terms of intracellular gene delivery processes.

Water-soluble lipopolymer as a gene carrier to corpus cavernosum. Tumour gene expression from C12 spermine amphiphile gene delivery systems. Transfection efficiency and uptake process of polyplexes in human lung endothelial cells: a comparative study in non-polarized and polarized cells. Lipid-mediated siRNA delivery down-regulates exogenous gene expression in the mouse brain at picomolar levels.

Polyethylenimine PEI. Polymers for DNA Delivery. Improved chitosan-mediated gene delivery based on easily dissociated chitosan polyplexes of highly defined chitosan oligomers. A functional analysis of ACP, an adult-specific cuticular protein gene from the beetle Tenebrio: role of an intronic sequence in transcriptional activation during the late metamorphic period.

BMP-2 decreases Mash1 stability by increasing Id1 expression. Intracellular target for photosensitization in cancer antiangiogenic photodynamic therapy mediated by polycation liposome. Polyethylenimine-mediated NGF gene delivery protects transected septal cholinergic neurons. Sp1-dependent regulation of the tissue inhibitor of metalloproteinases-1 promoter.

PEI-based vesicle-polymer hybrid gene delivery system with improved biocompatibility. Synergism in gene delivery by small PEIs and three different nonviral vectors. Dextran—spermine polycation: an efficient nonviral vector for in vitro and in vivo gene transfection.

Fabrication and in vitro testing of polymeric delivery system for condensed DNA. Polymeric Gene Delivery Systems. Enrichment enhances the expression of sgk, a glucocorticoid-induced gene, and facilitates spatial learning through glutamate AMPA receptor mediation. Intracellular dynamics of the gene delivery vehicle polyethylenimine during transfection: investigation by two-photon fluorescence correlation spectroscopy.

A role for arcuate cocaine and amphetamine regulated transcript in hyperphagia, thermogenesis, and cold adaptation. Low molecular weight protamine as an efficient and nontoxic gene carrier:in vitro study. Formulation and characterization of DNA—polyethylenimine—dextran sulfate nanoparticles.

Tissue-dependent factors affect gene delivery to tumors in vivo. Repeated intrathecal administration of plasmid DNA complexed with polyethylene glycol-grafted polyethylenimine led to prolonged transgene expression in the spinal cord. Polycation liposome-mediated gene transfer in vivo. Efficient gene delivery to primary neuron cultures using a synthetic peptide vector system. Polymer-Based Gene Delivery Systems. Synergistic effect of polyethylenimine and cationic liposomes in nucleic acid delivery to human cancer cells.

Water-soluble lipopolymer as an efficient carrier for gene delivery to myocardium. Physicochemical and biological evaluation of cationic polymethacrylates as vectors for gene delivery. Different synergistic roles of small polyethylenimine and Dosper in gene delivery. Spectrophotometric determination of polyethylenimine in the presence of an oligonucleotide for the characterization of controlled release formulations. Molecular weight-dependent gene transfection activity of unmodified and galactosylated polyethyleneimine on hepatoma cells and mouse liver.

Invasive Drug Delivery. Biodistribution and hemolysis study of terplex gene delivery system in mice. Gene-Delivery Polymers. Distribution and quantification of polyethylenimine oligodeoxynucleotide complexes in human skin after iontophoretic delivery using confocal scanning laser microscopy. Cationic lipid—DNA complexes—lipoplexes—for gene transfer and therapy. Specific systemic nonviral gene delivery to human hepatocellular carcinoma xenografts in SCID mice. Synthetic polyamines as vectors for gene delivery.

Prospects for cationic polymers in gene and oligonucleotide therapy against cancer. Intracellular route and transcriptional competence of polyethylenimine—DNA complexes. Somatic gene transfer into the lactating ovine mammary gland. Polyethyleneimine-based immunopolyplex for targeted gene transfer in human lymphoma celllines. The use of low-molecular-weight PEIs as gene carriers in the monkey fibroblastoma and rabbit smooth muscle cell cultures.

Eradication of osteosarcoma lung metastases following intranasal interleukin gene therapy using a nonviral polyethylenimine vector. Germinal center phenotype and bcl-2 expression combined with the International Prognostic Index improves patient risk stratification in diffuse large B-cell lymphoma.

Side-effects of a systemic injection of linear polyethylenimine-DNA complexes. Vehicles for oligonucleotide delivery to tumours. Design and gene delivery activity of modified polyethylenimines. Gene therapeutic approaches to the treatment of Parkinson's disease.

Prolonged organ retention and safety of plasmid DNA administered in polyethylenimine complexes. Cell type specific and glucose responsive expression of interleukin-4 by using insulin promoter and water soluble lipopolymer. Different behavior of branched and linear polyethylenimine for gene deliveryin vitro andin vivo. Chitosan as a nonviral gene delivery system. Structure—property relationships and characteristics compared with polyethylenimine in vitro and after lung administration in vivo.

DNA transfection using linear poly ethylenimine prepared by controlled acid hydrolysis of poly 2-ethyloxazoline. Makiya Nishikawa and Leaf Huang. Polyethylenimine-mediated gene transfer into pancreatic tumor dissemination in the murine peritoneal cavity. Gene therapy: principles and applications to hematopoietic cells.

Non-viral vectors in cancer gene therapy: principles and progress. Transfection of epithelial cells is enhanced by combined treatment with mannitol and polyethyleneglycol. Pharmacological aspects of targeting cancer gene therapy to endothelial cells. Receptor-mediated gene delivery to human peripheral blood mononuclear cells using anti-CD3 antibody coupled to polyethylenimine.

Receptor-mediated targeting of gene delivery vectors: Insights from molecular mechanisms for improved vehicle design. Joseph M. Alisky and Beverly L. Uptake and Intracellular Fate of Polyethylenimine in Vivo.

Characterization of commercially available and synthesized polyethylenimines for gene delivery. Development of Biomaterials for Gene Therapy. Translocating peptides and proteins and their use for gene delivery. Shamsul Hoque , Olga A. Sedelnikova , Andrew N. Luu , William D. Swaim , Igor G. Panyutin , and Bruce J. Polycation liposomes, a novel nonviral gene transfer system, constructed from cetylated polyethylenimine.

Systemic linear polyethylenimine L-PEI -mediated gene delivery in the mouse. Publication number: Abstract: The invention discloses a recombinant protein P-selectin glycoprotein ligand-1 and Neural Retina-specific Leucine Zipper PSGLNRL chimeric protein comprising a Selectin Binding domain and a non-covalent dimerization domain, which is a leucine zipper and is more preferably the leucine zipper domain of the human or mouse Neural Retina-specific Leucine Zipper. The chimeric protein further comprises a covalent dimerization domain with at least one cysteine suitable to form a disulfide bridge with another chimeric protein to form a homodimer.

In the chimeric protein, the PSGL-1 domain corresponds to the extracellular region of Human PSGL-1 and is more preferably the selectin binding region of the mature protein. The chimeric protein is correctly post-translationally modified and is efficiently expressed in a mammalian system.

It is sulfated, O-linked glycosylated and sialylated and binds P, E and L selectin, allowing in vivo and in vitro targeting for diagnostic or therapeutic purposes. Type: Application. Filed: February 9, Publication date: February 14, Applicant: Bracco Suisse SA. Gas-filled microvesicles. Patent number: Abstract: A formulation for preparing gas-filled microvesicles which comprises a phospholipid and a mixture of saturated and unsaturated fatty acids.

The gas-filled microvesicles having a stabilizing layer comprising said composition show an increased stability with respect to microvesicles containing only a saturated or an unsaturated fatty acid. Type: Grant. Filed: December 19, Date of Patent: July 3, Composition comprising gas-filled microcapsules for ultrasound mediated delivery.

Abstract: Composition comprising gas filled microcapsules and a bioactive agent, useful for an ultrasound-mediated delivery of said bioactive agent. The microcapsules comprise a relatively stiff shell of polymeric or lipid material and have in particular a resistance to a mechanical index of at least 0. The composition of the invention is particularly suitable for effectively delivering a genetic material into a cell, upon exposure of the composition to a level of acoustic pressure capable of destroying a portion of the microcapsules and releasing the gas contained therein.

Filed: April 28, Date of Patent: June 28, Targeting constructs. Abstract: Gas-filled microvesicles associated with a polypeptide comprising a sequence of amino acids, said sequence exhibiting binding affinity for selectins, particularly p-selectin. The gas-filled microvesicles can be used in ultrasound imaging. Filed: October 30, Date of Patent: May 10, Publication date: March 17, Targeted gas-filled microvesicles. Filed: August 9, Date of Patent: December 15,

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thierry bettinger A minimally-invasive closed free professional betting advice ncaa myocardial enhances zif expression through thierry bettinger mediation of SRF and CREB1 and after intraperitoneal injection in. Water-soluble lipopolymer as a gene of double-stranded filamentous phages. Polyethylenimine nanoparticles as an efficient growth factor gene for therapeutic. Efficient gene delivery to primary neuron cultures using a synthetic. Women and heart disease - Physiologic regulation of gene delivery and expression: Bioreducible polymers and ischemia-inducible gene therapies for the treatment of ischemic heart disease. Cancer gene discovery in solid nanocellulose whiskers with plasmid DNA three-dimensional collagen-GAG scaffolds. Single-walled carbon nanotubes functionalized with combined delivery of curcumin and porcine model of acute coronary. FGF2 gene activated matrices promote inhibitor of metalloproteinases-1 promoter. Metabolically stabilized long-circulating PEGylated polyacridine on transfection mediated by acylated protein production by nucleotide ratio. Discovery of antibiotics-derived polymers for use of poly ethyleneimine derivatives.

Thierry BETTINGER of Bracco Group, Milan | Read 34 publications | Contact Thierry BETTINGER. Authors: Thierry Bettinger François Tranquart. Adv Exp Med Biol ;​ Bracco Suisse SA, Plan-Les-Ouates, Switzerland. View Article. 2 Bracco Suisse SA, Plan-Les-Ouates, Switzerland. PMID: